China Science

OBJECTIVE: To evaluate the efficacy of INFORM HPV using the SurePathcollection method in women whose Pap tests indicated abnormal results. STUDY DESIGN: Ninety-twowomen from the gynecology clinics at The University of Texas M.D. Anderson Cancer Center who had Paptests and underwent follow-up biopsies were selected for the study. This included 51 women withatypical squamous cells of undetermined significance (ASCUS), 23 women with low-grade squamousintraepithelial lesion (LSIL), 15 women with high-grade squamous intraepithelial lesion (HSIL) and 3women with negative Pap results. The INFORM HPV, an in situ hybridization assay, testing foroncogenic types of HPV was performed, and the results were compared with follow-up biopsies.RESULTS: The positive rate of the INFORM HPV increased with higher grades of cytology diagnoses. Thesensitivity of the INFORM HPV testing for predicting high-grade cervical intraepithelial neoplasia(CIN 2/3) also increased with higher grades of cytology diagnoses. A negative predictive value (NPV)of 94.9% and a specificity of 80.4% for predicting CIN 2/3 were observed in the ASCUS group.CONCLUSION: Using SurePath Pap specimens, the INFORM HPV lacks sufficient sensitivity and NPV forpredicting CIN 2/3 in women with ASCUS. Therefore, use of the test as a triage tool is limited.

Guo,M　Patel,SJ　Chovanec,M　Jan,YJ　Tarco,E　Bevers,TB　Anderson,K　Sneige,N
Departments of Pathology, The University of Texas M.D. Anderson CancerCenter, Houston, Texas 77030-4009, USA.

Adult　 Aged　 Alphapapillomavirus　 Biopsy Cervical Intraepithelial Neoplasia