Characterization of the first Polish isolate of Neospora caninum from cattle
Neospora caninum was isolated from the brain of an apparently healthy calfborn to a seropositive cow. The calf was killed 12 hrs after birth and homogenate of its brain wasinoculated into Vero cells. Neospora-like tachyzoites were detected 66 days later. The identity ofthe parasite was confirmed by polymerase chain reaction amplification of N. canium-specificfragments using Np21 and Np6 primers. This first Polish isolate of N. caninum was designatedNC-PolB1.
Gozdzik, K　Cabaj, W
Polish Acad Sci, W Stefanski Inst Parasitol, PL-00818 Warsaw, Poland
Neospora caninum　 isolation　 PCR　 ELISA　 Western blot　 calf　 ANTIBODIES　INFECTION BRAIN DOGS
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